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| 产品名称： | ZR-75-30 |
|---|---|
| 商品货号： | TS210623 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland/breast; derived from metastatic site: ascites |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ductal carcinoma |
| Age： | 47 years |
| Gender： | female |
| Ethnicity： | Black |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 40% of cells. The rate of cells with higher ploidies was 1.2%. Sixteen marker chromosomes were found in most cells. |
| Images： |  |
| Derivation： | ZR-75-30 was derived from malignant ascites fluid from a 47-year-old premenopausal Black woman with infiltrating ductal carcinoma. xad |
| Clinical Data： | 47 years
Black
female |
| Comments： | These cells have been characterized to be human, non-HeLa, malignant mammary epithelium in origin. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 10,11 D16S539: 9 D5S818: 12,13 D7S820: 11,12 THO1: 8,9 TPOX: 10,11 vWA: 18,19 |
| Population Doubling Time： | 110 hrs |
| Name of Depositor： | LW Engel |
| Deposited As： | Homo sapiens |
| References： | Engel LW, Young NA. Human breast carcinoma cells in continuous culture: a review. Cancer Res. 38: 4327-4339, 1978. PubMed: 212193 Engel LW, et al. Establishment and characterization of three new continuous cell lines derived from human breast carcinomas. Cancer Res. 38: 3352-3364, 1978. PubMed: 688225 Littlewood-Evans AJ, et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: 9393764 |