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微信小章| 产品名称： | ZEM2S |
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| 商品货号： | TS210624 |
| Organism： | Danio rerio, zebrafish |
| Tissue： | embryo |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | embryo; blastula |
| Applications： | These cells provide an in vitro assay system for the comparison of enhancer/promotor activities in early stage zebrafish embryos.
The cells may provide an in vitro assay system for the study of extracelluar factors which induce and regulate cell differentiation.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | ZEM2S was derived from the ZEM2 cell line that had been established from zebrafish embryos using a complex growth medium supplemented with insulin, trout embryo extract, trout and fetal bovine sera.
ZEM2S cells were derived from ZEM2 in 1993 by selection for growth in a basal nutrient medium supplemented with 5 to 10% heat-inactivated fetal bovine serum. |
| Comments： | Medium conditioned by cells from the buffalo rat liver cell line available as BRL 3A (see ATCC CRL-1442). Both transient and stable expression of foreign genes have been demonstrated in transfected zebrafish blastula derived cell cultures. A culture submitted to the ATCC in October 1994 was found to be contaminated with mycoplasma, and progeny were cured by a 21 day treatment with BM Cycline. |
| Complete Growth Medium： | 50% Leibovitzs L-15 medium (ATCC 30-2008) 35% Dulbeccos Modified Eagles medium, high glucose (GIBCO 12100) 15% F12 medium (GIBCO 21700) The above are all without sodium bicarbonate Supplemented with: 0.18 g/L sodium bicarbonate 15 mM HEPES 10% heat-inactivated fetal bovine serum |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 85%; additional HI-FBS, 10%, DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 28°C
Max Temperature: 29°C
Min Temperature: 26°C |
| Name of Depositor： | DW Barnes |
| Deposited As： | Brachydanio rerio |
| Year of Origin： | 1993 |
| References： | Collodi P, et al. Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues. Cell Biol. Toxicol. 8: 43-61, 1992. PubMed: 1591622 Ghosh C, Collodi P. Culture of cells from zebrafish (Brachydanio rerio) blastula-stage embryos. Cytotechnology 14: 21-26, 1994. PubMed: 7765109 Bradford CS, et al. Cell Cultures from zebrafish embryos and adult tissues. J. Tissue Culture Methods 16: 99-107, 1994. |