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| 产品名称： | Y-3P |
|---|---|
| 商品货号： | TS210628 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k. It cross-reacts with all rat I-A like molecules. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against I-A; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against I-A; cytotoxic |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with spleen cells from (BALB/c X C57BL/6)F1 mice and boosted with spleen cells from BALB/c mice immunized with either BALB.B or C57BL/6 T cell blasts. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with I-A b, f, p, q, r, s, u, and v haplotypes and weakly with I-A k. It cross-reacts with all rat I-A like molecules. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640 with 10 mM HEPES, 95%; fetal bovine serum, 5%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a |
| Name of Depositor： | C Janeway |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Janeway CA Jr., et al. Monoclonal antibodies specific for Ia glycoproteins raised by immunization with activated T cells: possible role of T cellbound Ia antigens as targets of immunoregulatory T cells. J. Immunol. 132: 662-667, 1984. PubMed: 6228596 |