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| 产品名称： | Y-17 |
|---|---|
| 商品货号： | TS210632 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 BALB/c (B cell); BALB/c (myeloma) |
| Applications： | The antibody reacts with a conformational or combinatorial determinant on Ea:Eb complexes (Ae:Ea) of the b, k, r, s and v haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against I-E; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against I-E; cytotoxic |
| Comments： | Animals were immunized with Con A stimulated spleen cells from B10.A(5R) mice. Spleen cells were fused with Sp2/0 myeloma cells. The antibody reacts with a conformational or combinatorial determinant on Ea:Eb complexes (Ae:Ea) of the b, k, r, s and v haplotypes. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Isotype： | IgG2b |
| Name of Depositor： | C Janeway |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Lerner EA, et al. Monoclonal antibody against an Ir gene product. J. Exp. Med. 152: 1085-1101, 1980. PubMed: 6158546 |