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微信小章| 产品名称： | YAC-1 |
|---|---|
| 商品货号： | TS210635 |
| Organism： | Mus musculus, mouse |
| Cell Type： | Moloney murine leukemia virus (Mo-MuLV) induced |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | A/Sn |
| Applications： | This cell line can be used to assay of NK cell. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 cells/mL and maintain between 2 X 105 and 2 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37C |
| Name of Depositor： | JY Djeu |
| Deposited As： | Mus musculus |
| References： | Kiessling R, et al. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur. J. Immunol. 5: 112-117, 1975. PubMed: 1234049 Cikes M, et al. Progressive loss of H-2 antigens with concomitant increase of cell- surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. J. Natl. Cancer Inst. 50: 347-362, 1973. PubMed: 4573851 Okazaki IJ, et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J. Biol. Chem. 271: 22052-22057, 1996. PubMed: 8703012 Kiessling R, et al. "Natural" killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Eur. J. Immunol. 5: 117-121, 1975. PubMed: 1086218 YAC-1 is a lymphoma which was induced by inoculation of the Moloney leukemia virus (MLV) into a newborn A/Sn mouse. The cells are sensitive to the action of natural killer (NK) cells and are useful in assays of NK cell activity. |