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微信小章| 产品名称： | xrs5 |
|---|---|
| 商品货号： | TS210641 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | xrs5 is a X-ray sensitive Chinese Hamster Ovary mutant cell line which was derived from CHO-K1 cells by treating the cells with ethyl methanesulphonate and subsequent growth in agar. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | xrs5 is a X-ray sensitive Chinese Hamster Ovary mutant cell line which was derived from CHO-K1 cells by treating the cells with ethyl methanesulphonate and subsequent growth in agar. |
| Clinical Data： | female |
| Comments： | xrs5 is a X-ray sensitive Chinese Hamster Ovary mutant cell line which was derived from CHO-K1 cells by treating the cells with ethyl methanesulphonate and subsequent growth in agar. These cells belong to X-ray complementation group 5 and are mutant in the p86 subunit of the Ku autoantigen. Ku has been shown to be involved in DNA double-strand break repair and V(D)J recombination. The cells will revert to wild type on treatment with azacytidine. |
| Complete Growth Medium： | Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:6 to 1:10 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | G Chu |
| References： | Jeggo PA, Kemp LM. X-ray-sensitive mutants of Chinese hamster ovary cell line. Isolation and cross-sensitivity to other DNA-damaging agents. Mutat. Res. 112: 313-327, 1983. PubMed: 6197643 Rathmell WK, Chu G. Involvement of the Ku autoantigen in the cellular response to DNA double-strand breaks. Proc. Natl. Acad. Sci. USA 91: 7623-7627, 1994. PubMed: 8052631 Rathmell WK, Chu G. A DNA end-binding factor involved in double-strand break repair and V(D)J recombination. Mol. Cell. Biol. 14: 4741-4748, 1994. PubMed: 7516471 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. |