| 产品名称: | XS63 |
|---|---|
| 商品货号: | TS210645 |
| Organism: | Mus musculus, mouse |
| Cell Type: | B lymphocyte |
| Product Format: | frozen |
| Morphology: | lymphoblast |
| Culture Properties: | suspension |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease: | plasmacytoma; myeloma |
| Strain: | BALB/c |
| Applications: | The XS63 cell line was established from the IgA secreting BALB/c myeloma. The cells do not secrete or synthesize immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
| Derivation: | The XS63 cell line was established from the IgA secreting BALB/c myeloma. |
| Antigen Expression: | H-2d |
| Genes Expressed: | H-2d |
| Comments: | The XS63 cell line was established from the IgA secreting BALB/c myeloma. The cells do not secrete or synthesize immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium: | Dulbeccos modified Eagles medium, 90%; horse serum, 10%
|
| Subculturing: | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor: | D Schubert, K Horibata |
| Deposited As: | Mus musculus |
| References: | Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 Schubert D, Horibata K. Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. J. Mol. Biol. 38: 263-271, 1968. PubMed: 4180612 . . Cold Spring Harbor Symp. Quant. Biol. 32: 104, 1967. |