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微信小章| 产品名称： | Y-1 |
|---|---|
| 商品货号： | TS210647 |
| Organism： | Mus musculus, mouse |
| Tissue： | adrenal gland; tumor/cortex |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | male |
| Strain： | LAF1 |
| Applications： | This cell line is useful for the detection of LT toxin (American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992.), cholera toxin (U.S. Food & Drug Administration Detection of cholera enterotoxin (CT) and cytotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD AOAC International pp. 9.12-9.15, 1995), and verotoxin.xa0 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 41; range = 40 to 108. Stemline number is hyperdiploid. Karyotype stable within stemline number. Thirty-nine chromosomes with terminal centromeres plus 2 minute chromosomes; some cells with only l minute chromosome. The first chromosome is quite large and some of the smaller chromosomes are smaller than normal, but no specific structural abnormalities were detected. |
| Images： |  |
| Clinical Data： | male |
| Genes Expressed： | steroid hormones |
| Cellular Products： | steroid hormones |
| Virus Susceptibility： | Herpes simplex virus
Pseudorabies virus Vaccinia virus Vesicular stomatitis virus Human poliovirus 1 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: At least 2 times per week (or else hormone production will decrease) |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | G Sato |
| Deposited As： | Mus musculus |
| References： | Yasumura Y, et al. Clonal analysis of differentiated function in animal cell cultures. I. Possible correlated maintenance of differentiated function and the diploid karyotype. Cancer Res. 26: 529-535, 1966. PubMed: 5930699 American Public Health Association. Compendium of methods for the microbiological examination of foods. 3rd ed.Washington, DC: American Public Health Association; 1992. U.S. Food & Drug Administration Detection of cholera enterotoxin (CT) and cytotoxin In: U.S. Food & Drug Administration Bacteriological analytical manual 8th ed.Gaithersburg, MD AOAC International pp. 9.12-9.15, 1995 Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682 Cohen AI, et al. Histologic and physiologic characteristics of hormone-secreting transplantable adrenal tumors in mice and rats. Am. J. Pathol. 33: 631-651, 1957. PubMed: 13444454 Cohen AI, et al. In vitro response of functional experimental adrenal tumors to corticotropin ACTH. Proc. Soc. Exp. Biol. Med. 95: 304-309, 1957. PubMed: 13441719 Rainey WE, et al. Adrenocortical cell lines. Mol. Cell Endocrinol. 228(1-2): 23-38, 2004. PubMed: 15541570 |