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微信小章| 产品名称： | WSS-1 WS-1 |
|---|---|
| 商品货号： | TS210648 |
| Organism： | Homo sapiens, human |
| Tissue： | kidney |
| Cell Type： | Transformed with adenovirus 5 DNA |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0xa0Cells contain Adenovirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | fetus |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was derived from the human embryonic kidney line, 293 (see ATCC CRL-1573). 293 cells were transfected with an expression plasmid containing cDNA encoding the rat GABA-A receptor alpha 1, beta 2 and gamma 2 subunits. Genomic integration of the transfected alpha and gamma subunit genes was confirmed; however, the beta subunit sequences were not detected in Northern and Southern blots. Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits). |
| Receptor Expression： | vitronectin; gamma-aminobutyric acid - A receptor (GABA-A, Type 1 BzR) |
| Genes Expressed： | gamma-aminobutyric acid (GABA) A receptor, alpha 1 GABRA1,gamma-aminobutyric acid (GABA) A receptor, alpha 1 |
| Comments： | Produces protein gamma-aminobutyric acid (GABA) A receptor, alpha 1
Handle as potentially biohazardous material under at least Biosafety Level 2 containment. Stable transfectant expressing a type I GABAA receptor (alpha1 and gamma2 subunits). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine modified to contain 4.5 g/L glucose, 1.5 g/L sodium bicarbonate and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
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| Subculturing： | Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze Medium: Culture medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 12,14 D16S539: 9,13 D5S818: 8 D7S820: 11,12 THO1: 7,9.3 TPOX: 11 vWA: 16,19 |
| Name of Depositor： | G Wong |
| Deposited As： | Homo sapiens |
| References： | Wong G, et al. Stable expression of type I gamma-aminobutyric acidA/benzodiazepine receptors in a transfected cell line. Mol. Pharmacol. 42: 996-1003, 1992. PubMed: 1336119 |