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微信小章| 产品名称： | WRL 68 |
|---|---|
| 商品货号： | TS210649 |
| Organism： | Homo sapiens, human |
| Tissue： | HeLa contaminant |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 2 xa0Cells contain Human Papilloma Virus (HPV) DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | A culture submitted to the ATCC in July of 1969 was found to be contaminated with mycoplasma, and was cured by treatment with Ciprofloxacin |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:2 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% Carbon dioxide (CO2) |
| STR Profile： | Amelogenin: X CSF1PO: 9,10 D13S317: 12,13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Isoenzymes： | G6PD, A |
| Name of Depositor： | Burroughs Wellcome Company |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| References： | Apostolov K. Cell lines. US Patent 3,935,066 dated Jan 27 1976 Gutierrez-Ruiz MC, et al. Expression of some hepatocyte-like functional properties of WRL-68 cells in culture. In Vitro Cell. Dev. Biol. 30A: 366-371, 1994. PubMed: 7522099 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |