微信小陈
微信小章| 产品名称： | WS1 |
|---|---|
| 商品货号： | TS210651 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 12 week gestation |
| Gender： | female |
| Ethnicity： | Black |
| Storage Conditions： | liqudi nitrogen vapor phase |
| Derivation： | WS1 was derived from normal human skin by RJ Hay. |
| Clinical Data： | 12 week gestation Black female |
| Comments： | WS1 cells have a doubling potential of 67 population doublings. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liqudi nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,13 D13S317: 12 D16S539: 10,11 D5S818: 13 D7S820: 9,10 THO1: 8,10 TPOX: 8,9 vWA: 17,18 |
| Population Doubling Capacity： | WS1 cells have a doubling potential of 67 population doublings. |
| Name of Depositor： | RJ Hay |
| Deposited As： | Homo sapiens |
| References： | Huang LC, et al. Sensitivity and selectivity of the DNA damage sensor responsible for activating p53-dependent G1 arrest. Proc. Natl. Acad. Sci. USA 93: 4827-4832, 1996. PubMed: 8643488 Corfield VA, Hay RJ. Effects of cystine or glutamine restriction on human diploid fibroblasts in culture. In Vitro 14: 787-794, 1978. PubMed: 721103 |
| Cross References： | Nucleotide (GenBank) : U19758 Human chromosome 7 beta-actin region clone pCC2Dee. Nucleotide (GenBank) : U19757 Human chromosome 7 beta-actin region clone pCC10Jhb. |