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| 产品名称： | X16C8.5 X16C |
|---|---|
| 商品货号： | TS210654 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | reticulum cell sarcoma |
| Strain： | BALB/cAnN |
| Applications： | Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | near tetraploid; modal number = 78; range = 39 to 84 |
| Derivation： | Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse. |
| Antigen Expression： | I-A+; H-2d |
| Receptor Expression： | Fc |
| Genes Expressed： | immunoglobulin (both surface and secreted, sIg+),I-A+; H-2d,Expression of I-A and H-2 is increased by treatment with interferon. |
| Cellular Products： | immunoglobulin (both surface and secreted, sIg+) |
| Tumorigenic： | No |
| Effects： | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | Derived from a spontaneous reticulum cell neoplasm found in an old BALB/cAnN mouse. Expression of I-A and H-2 is increased by treatment with interferon. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Isotype： | IgM |
| Population Doubling Time： | 18 hrs |
| Name of Depositor： | KJ Kim |
| Deposited As： | Mus musculus |
| References： | Kim KJ, et al. Establishment and characterization of BALB/c lymphoma lines with B cell properties. J. Immunol. 122: 549-554, 1979. PubMed: 310843 Kim KJ, et al. Characterization of T-cell-soluble factors modulating the expression of Ia and H-2 antigens on BALB/c B lymphoma cell lines. Cell. Immunol. 76: 253-267, 1983. PubMed: 6404559 |