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微信小章| 产品名称： | XB-2 |
|---|---|
| 商品货号： | TS210655 |
| Organism： | Mus musculus, mouse |
| Cell Type： | epithelial keratinocyte |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | teratoma |
| Strain： | CXBGB/By |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | XB-2 cells can undergo a process of terminal differention similar to keratinocytes. When cultured at low cell density as adherent cells, few cells differentiate. When placed in suspension culture, however, cultures demonstrate colony forming ability decrease, nuclear pyknosis and other features of keratinocyte differentiation. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Plate ATCC 56-X, irradiated STO, in the flasks 2 to 24 hours before use.xa0
Subcultivation Ratio: See subcultivation information above Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley - Liss, N.Y., 2005. |
| Cryopreservation： | Freeze medium: Complete culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | Massachusetts Institute of Technology |
| Deposited As： | Mus musculus |
| U.S. Patent Number： | |
| References： | Green H, Rheinwald JG. Process for serially culturing keratinocytes. US Patent 4,016,036 dated Apr 5 1977 Morrissey JH, Green H. Differentiation-related death of an established keratinocyte line in suspension culture. J. Cell. Physiol. 97: 469-476, 1978. PubMed: 730781 Stevens LC. The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev. Biol. 21: 364-382, 1970. PubMed: 5436899 XB-2 was isolated from a transplantable mouse teratoma (No. 69691) established by L. C. Stevens. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |