微信小陈
微信小章| 产品名称： | XC1.5/51 |
|---|---|
| 商品货号： | TS210656 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | plasmacytoma; myeloma |
| Strain： | C3H |
| Applications： | The cells do not synthesize or secrete immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
| Karyotype： | near tetraploid |
| Derivation： | A derivative of C1.18 (ATCC TIB-11). |
| Comments： | A derivative of C1.18 (ATCC TIB-11). The cells do not synthesize or secrete immunoglobulin. The cells do not express PC.1 or H-2 k. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium, 90%; horse serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor： | K Horibata, D Schubert |
| Deposited As： | Mus musculus |
| References： | Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883 Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714 Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914 Schubert D, Horibata K. Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. J. Mol. Biol. 38: 263-271, 1968. PubMed: 4180612 Schubert D, Cohn M. Immunoglobulin biosynthesis. 3. Blocks in defective synthesis. J. Mol. Biol. 38: 273-288, 1968. PubMed: 4180613 J. Natl. Cancer Inst. 19: 371, 1957. Kimmel CB. On the RNA in cultured myeloma cells producing immunoglobulin. Biochim. Biophys. Acta 182: 361-374, 1969. PubMed: 4183552 |