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微信小章| 产品名称： | XC |
|---|---|
| 商品货号： | TS210657 |
| Organism： | Rattus norvegicus, rat |
| Cell Type： | virus induced |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Rous sarcoma |
| Strain： | White-Weston |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Virus Resistance： | poliovirus 1 |
| Comments： | Syncytial plaques are formed when cells are infected with MuLV. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | JW Hartley |
| Deposited As： | Rattus sp. |
| References： | Rowe WP, et al. Plaque assay techniques for murine leukemia viruses. Virology 42: 1136-1139, 1970. PubMed: 4099080 Klement V, et al. Mixed culture cytopathogenicity: a new test for growth of murine leukemia viruses in tissue culture. Proc. Natl. Acad. Sci. USA 63: 753-758, 1969. PubMed: 4186808 . . Folia Biol. 8: 221-228, 1962. . . Folia Biol. 7: 46-60, 1961. . . Folia Biol. 9: 77-81, 1963. . . Nature 186: 980-981, 1960. Simkovic D, et al. An in vitro system for the detection of the Rous sarcoma virus in the cells of the rat tumour XC. Neoplasma 9: 104-106, 1962. PubMed: 13913043 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |