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| 产品名称： | WR19L |
|---|---|
| 商品货号： | TS210659 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | BALB/c |
| Applications： | The line was derived from the WR19 tumor induced by Abelson murine leukemia virus (see also ATCC TIB-70) but does not produce Abelson virus. The cells are negative for surface immunoglobulin (sIg-) and Ia (Ia-). The line does not pinocytose neutral red, phagocytose zymosan or latex beads and does not secrete lysozyme. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The line was derived from the WR19 tumor induced by Abelson murine leukemia virus (see also ATCC TIB-70) but does not produce Abelson virus. |
| Antigen Expression： | H-2d; Thy-1.2 |
| Genes Expressed： | Moloney murine leukemia virus,H-2d; Thy-1.2 |
| Cellular Products： | Moloney murine leukemia virus |
| Comments： | The line was derived from the WR19 tumor induced by Abelson murine leukemia virus (see also ATCC TIB-70) but does not produce Abelson virus. The cells are negative for surface immunoglobulin (sIg-) and Ia (Ia-). The line does not pinocytose neutral red, phagocytose zymosan or latex beads and does not secrete lysozyme. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Name of Depositor： | WC Raschke |
| Deposited As： | Mus musculus |
| References： | Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198 |