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| 产品名称： | WR21 |
|---|---|
| 商品货号： | TS210662 |
| Organism： | Mus musculus, mouse |
| Tissue： | submandibular salivary gland |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | adult |
| Gender： | male |
| Strain： | subline 69-2 (C57BL/6, SJL transgenic) |
| Applications： | WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989. |
| Clinical Data： | WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989. male |
| Oncogene： | c-H-ras + (human) |
| Genes Expressed： | vimentin; fibronectin,c-H-ras + (human) |
| Cellular Products： | vimentin; fibronectin |
| Tumorigenic： | Yes |
| Effects： | Yes, the cells give rise to aggressive, highly anaplastic solid tumors when injected subcutaneously into athymic nude mice |
| Comments： | WR21 cells were cultured from a salivary tumor taken from an adult male wap-ras subline 69=2 transgenic mouse in the fall of 1989. These mice express an activated human c-Ha-ras gene and develop adenocarcinomas arising from the submandibular gland and/or mammary progenitor cells by one year of age. Subline 69-2 has a random bred genetic background of two inbred mouse strains, C57BL/6 and SJL. The cells were subcloned in 1990. It is impossible to ascertain the original cell type with any certainty. They express fibronectin and vimentin but not cytokeratin or desmin. WR21 cells have an activated human Ha-ras oncogene on the Y chromosome and express Asp-12 mutant ras protein. In nude mice with WR21 tumors, approximately 90% had lung metastases by the fifth week of tumor growth, but no tumors metastasized to the salivary gland. WR21 tumor growth was inhibited by cyclophosphamide, 5-fluorouracil, adriamycin, mitomycin C and actinomycin D but not methotrexate. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended Medium Renewal: Twice per week Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Name of Depositor： | LL Nielsen |
| Deposited As： | Mus musculus |
| References： | Nielsen LL, et al. Development of a nude mouse model of ras-mediated neoplasia using WR21 cells from a transgenic mouse salivary tumor. In Vivo 8: 295-302, 1994. PubMed: 7803707 |