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微信小章| 产品名称： | WEHI-13VAR |
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| 商品货号： | TS210668 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | mixed, adherent and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrosarcoma |
| Strain： | BALB/c |
| Applications： | This line provides a stable and highly sensitive bioassay system to detect and measure mouse and human natural and recombinant tumor necrosis factors (TNF alpha and lymphotoxin. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | WEHI-13VAR, is a variant of WEHI 164 clone 13 which has lost its sensitivity to Tumor Necrosis Factor (TNF) in the absence of actinomycin D. |
| Comments： | The WEHI-13VAR cell line was found to maintain its high sensitivity to TNF when the assay was performed in the presence of 500 ng/mL actinomycin D. This cell line is more sensitive to TNF alpha and lymphotoxin than L929 (ATCC CCL-1) or WEHI 164 (ATCC CRL-1751). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:4 to 1:6 Medium Renewal: Twice a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JA Armstrong |
| Deposited As： | Mus musculus |
| References： | Espevik T, Nissen-Meyer J. A highly sensitive cell line, WEHI 164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J. Immunol. Methods 95: 99-105, 1986. PubMed: 3782828 Abu-Khabar KS, et al. Type I interferons (IFN-alpha and -beta) suppress cytotoxin (tumor necrosis factor-alpha and lymphotoxin) production by mitogen-stimulated human peripheral blood mononuclear cell. J. Leukocyte Biol. 52: 165-173, 1992. PubMed: 1506772 Khabar KS, et al. WEHI-13VAR: a stable and sensitive variant of WEHI 164 clone 13 fibrosarcoma for tumor necrosis factor bioassay. Immunol. Lett. 46: 107-110, 1995. PubMed: 7590904 |