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| 产品名称： | WEHI-274.1 |
|---|---|
| 商品货号： | TS210669 |
| Organism： | Mus musculus, mouse |
| Cell Type： | monocyte |
| Product Format： | frozen |
| Morphology： | monocyte |
| Culture Properties： | suspension |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Abelson murine leukemia virus-induced tumor |
| Strain： | BALB/c |
| Applications： | The line expresses the MAC-1 antigen, does not express Ia and is not phagocytic. Tested and found negative for ectromelia virus (mousepox). |
| Antigen Expression： | MAC-1 antigen (CD11b) |
| Receptor Expression： | Fc |
| Genes Expressed： | MAC-1 antigen (CD11b) |
| Comments： | The line expresses the MAC-1 antigen, does not express Ia and is not phagocytic. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10%; 0.05 mM 2-mercaptoethanol
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Adherent cells can be dislodged by scraping. Start new cultures at 1 X 10 exp5 cells/ml and maintain at or below 1 X 10 exp6 cells/ml. |
| Name of Depositor： | NL Warner, LL Lanier |
| Deposited As： | Mus musculus |
| References： | . Immunobiology and immunotherapy of cancer. New York: Elsevier/North Holland; 1979. Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787 |