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微信小章| 产品名称： | WEHI-279 |
|---|---|
| 商品货号： | TS210670 |
| Organism： | Mus musculus, mouse |
| Tissue： | bone marrow |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, moderate to large multi-cell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | NZC |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | Expresses immunoglobulin on its surface only (surface IgM, kappa). It does not secrete any detectable immunoglobulin. |
| Cellular Products： | immunoglobulin |
| Comments： | Expresses immunoglobulin on its surface only (surface IgM, kappa). It does not secrete any detectable immunoglobulin. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively cultures can be established by centrifugation with subsequent resuspension at 5 x 104 to 1 x 105 viable cells/mL. Maintain cell density between 5 x 104 and 5 x 105 cells/mL.
Frozen cells may take longer than 2-3 days in complete culture medium to successfully recover from the frozen state.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 in air atmosphere. |
| Isotype： | IgM (surface); kappa light chain |
| Name of Depositor： | NL Warner, LL Lanier |
| Deposited As： | Mus musculus |
| References： | Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906 Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325 Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803 |