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微信小章| 产品名称： | WEHI-231 |
|---|---|
| 商品货号： | TS210671 |
| Organism： | Mus musculus, mouse |
| Cell Type： | B lymphocyte, immature |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension, multicell aggregates |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | B cell lymphoma |
| Strain： | (BALB/c x NZB)F1 |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Mineral oil induced tumor in (BALB/c x NZB)F1 mice |
| Genes Expressed： | immunoglobulin |
| Cellular Products： | immunoglobulin |
| Comments： | WEHI-231 cells do not secrete IgM into medium unless stimulated with lipopolysaccharide (0.01 to 3 μg/mL). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Optimum culture recovery from a frozen vial is in a T25 flask at a density between 2 to 5 X 105 viable cells/mL. If recovered at 5 X 105 viable cells/mL, media will need to be added to the culture within the first 3 days, before the density reaches 1 X 106 viable cells/mL, at which point the viability drops drastically.
After cells recover, expansions can be done at 1 X 105 viable cells/mL by adding media to decrease the cell concentration. This cell line forms tight clusters of viable cells accompanied by some single non-viable cells plus debris in suspension. With increased growth the clusters become larger. As the cell density nears 1 X 106 cells/mL, the single (non-clustered) and non-viable cells increase along with the amount of debris. At subculture, break up clusters by gentle pipetting. Established cultures can be grown in T75 flasks. Subcultivation Ratio: Add fresh medium every 2 to 3 days (depending on cell density)
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Isotype： | IgM , IgM (surface) |
| Population Doubling Time： | about 20 hours |
| Name of Depositor： | NL Warner, LL Lanier |
| Deposited As： | Mus musculus |
| References： | Boyd AW, et al. The regulation of growth and differentiation of a murine B cell lymphoma. I. Lipopolysaccharide induced differentiation. J. Immunol. 126: 2461-2465, 1981. PubMed: 6785355 Lanier LL, Warner NL. Cell cycle related heterogeneity of Ia antigen expression on a murine B lymphoma cell line:analysis by flow cytometry. J. Immunol. 126: 626-631, 1981. PubMed: 6969756 Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803 mineral oil induced tumor in (BALB/c x NZB)F1 mice To secrete IgM into the medium, these cells need to be stimulated with lipopolysaccharide (0.01 to 3 mcg/ml). |