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微信小章| 产品名称： | WEHI-3 |
|---|---|
| 商品货号： | TS210673 |
| Organism： | Mus musculus, mouse |
| Tissue： | peripheral blood |
| Product Format： | frozen |
| Culture Properties： | suspension (some adherent cells) |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | leukemia |
| Strain： | BALB/c |
| Applications： | Tested and found negative for ectromelia virus (mousepox). The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Receptor Expression： | complement (C3) |
| Genes Expressed： | lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3) |
| Cellular Products： | lysozyme; granulocyte colony stimulating activity (CSA); interleukin-3 (interleukin 3, IL-3) |
| Comments： | The growth of WEHI-3 is inhibited by 4 ng/mL LPS and blocked by higher concentrations.
Dextran sulfate at 30 to 40 µg/mL also inhibits growth.
Latex beads are phagocytized but are not toxic.
Zymosan and BCG are phagocytized and block growth.
The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 105 viable cells/mL.
Maintain cell density between 2 X 105 and 2 X 106 viable cells/mL. Adherent cells may be harvested by scraping.
Medium Renewal:xa0Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | M Cohn |
| Deposited As： | Mus musculus |
| References： | Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890 Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031 Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922 McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720 Boring L, et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787 |