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| 产品名称： | WERI-Rb-1 |
|---|---|
| 商品货号： | TS210674 |
| Organism： | Homo sapiens, human |
| Tissue： | eye, retina |
| Cell Type： | retinoblastoma |
| Product Format： | frozen |
| Morphology： | grape-like clusters of round cells |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | retinoblastoma |
| Age： | 1 year |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1,?), t(3p,5q), der(3)t(?q29;?), t(3q,?), 5q+, i(6p), t(7q,?), 9q+, t(10q,21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations |
| Derivation： | The WERI-Rb-1 line is one of two human retinoblastoma cell lines established in 1974 by R.M. McFall and T.W. Sery. |
| Clinical Data： | 1 year Caucasian female |
| Tumorigenic： | Yes |
| Effects： | Yes, in rabbits |
| Comments： | The cells survive culturing in Difco Bacto-Agar but do not form colonies. Scanning electron microscopy reveals some variation in the number and frequency of surface blebs, lamellipodia and microvilli. The line is of interest in studies of cell differentiation, animal models of tumor therapy and biochemical evaluations. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 3 X 105 viable cells/mL. Maintain cell density between 1 X 105xa0and 1 to 2 X 106xa0viable cells/mL.
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: 5% CO2 in air recommended Temperature: 37°C |
| Isoenzymes： | ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 0 |
| Name of Depositor： | TW Sery |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1974 |
| References： | McFall RC, et al. Characterization of a new continuous cell line derived from a human retinoblastoma. Cancer Res. 37: 1003-1010, 1977. PubMed: 844036 McFall RC, et al. Scanning electron microscopic observation of two retinoblastoma cell lines. Cancer Res. 38: 2827-2835, 1978. PubMed: 679190 Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024 |