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| 产品名称： | Wgd5 |
|---|---|
| 商品货号： | TS210675 |
| Organism： | Mus musculus, mouse |
| Tissue： | connective tissue |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Leukemia |
| Applications： | transfection host Helper cell line for packaging retroviral vectors such as those released from lambdaZd31, lambdaZd32, and lambdaZd35 (ATCC 37563-5, respectively). |
| Derivation： | The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus). |
| Genes Expressed： | retrovirus vector (Moloney murine leukemia virus |
| Cellular Products： | retrovirus vector (Moloney murine leukemia virus |
| Comments： | The Wgd5 cell line contains a mutant of the Moloney murine leukemia virus (MoMuLV) that is useful for packaging recombinant retroviral DNA into infectious virus particles. The line was derived from the WOP cell line (a 3T3 derivative transformed by an origin defective polyomavirus). Wgd5 was selected as a gpt+ clone after cotransformation of WOP with pSV2gpt and a packaging mutant of MoMuLV. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 0.25 mg/ml xanthine, 0.015 mg/ml hypoxanthine, 0.01 mg/ml thymidine, 0.002 mg/ml aminopterin and 0.015 mg/ml mycophenolic acid, 90%; fetal bovine serum, 10%
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, rinse and remove trypsin. Allow the cells to sit at 37C until they begin to detach (about 3 to 5 minutes), add fresh medium, aspirate and dispense into new flasks. Subculture before the cells become confluent. If allowed to become confluent, the cells will slough off and die. |
| Name of Depositor： | AJ Murphy |
| Deposited As： | Mus musculus |
| References： | Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791 |