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微信小章| 产品名称： | WCH-17 |
|---|---|
| 商品货号： | TS210679 |
| Organism： | Marmota monax, woodchuck, Eastern |
| Tissue： | liver |
| Cell Type： | woodchuck hepatitis B virus (WHV) induced |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain Hepadnavirus
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma |
| Age： | adult |
| Applications： | WCH-17 are useful for biological assays of woodchuck interferon, and can be used to propagate HVM. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Comments： | WCH-17 are useful for biological assays of woodchuck interferon, and can be used to propagate HVM. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | B Fernie |
| Deposited As： | Marmota monax |
| References： | Schechter EM, et al. Characterization of a herpesvirus isolated from woodchuck hepatocytes. J. Gen. Virol. 69: 1591-1599, 1988. PubMed: 2839596 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |