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| 产品名称： | WAPS 12.2 |
|---|---|
| 商品货号： | TS210680 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human P-selectin (CD62P) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human P-selectin (CD62P) |
| Comments： | Animals were immunized with P-selectin transfected mouse L1-2 pre-B cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody blocks leukocyte adhesion to P-selectin and to platelets. It recognizes P-selectin of a variety of mammals. |
| Complete Growth Medium： | Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate supplemented with 0.01 mM non-essential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG1 |
| Name of Depositor： | EC Butcher |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Jutila MA, et al. Cell surface P- and E-selectin support shear-dependent rolling of bovine gamma/delta T cells. J. Immunol. 153: 3917-3928, 1994. PubMed: 7523509 |