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微信小章| 产品名称： | W6/32 |
|---|---|
| 商品货号： | TS210682 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | Spleen, tonsils |
| Cell Type： | Hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | Lymphoblast-like |
| Culture Properties： | Suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | BALB/c (B cell); BALB/c (myeloma) |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Animals were immunized with cells from human tonsils.xa0Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Comments： | The antibody reacts with monomorphic determinants on HLA A,B,C molecules. The antibody is cytotoxic. The antibody cross-reacts with ape, monkey and cow cells. It does not react with rabbit cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.xa0
Medium Renewal:xa0Every 2 to 3 days |
| Cryopreservation： | 95% FBS ; 5% DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 |
| Isotype： | IgG2a |
| Antigenic Determinant： | HLA-A, B, C |
| Name of Depositor： | P Parham |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Brodsky FM, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128: 129-135, 1982. PubMed: 6172474 Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938 Parham P, et al. Use of a monoclonal antibody (W6/32) in structural studies of HLA- A,B,C, antigens. J. Immunol. 123: 342-349, 1979. PubMed: 87477 Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |