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微信小章| 产品名称： | W-20-17 W-20 clone 17 |
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| 商品货号： | TS210683 |
| Organism： | Mus musculus, mouse |
| Tissue： | bone marrow; stroma |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This cell line is used to evaluate the bioactivity of recombinant human bone morphogenetic protein-2 (rhBMP-2). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Cells from W++ mouse bone marrow were cloned by limiting dilution to obtain the W-20-17 cell line.xa0Ref |
| Genes Expressed： | alkaline phosphatase induced by recombinant human bone morphogenetic protein-2 (rhBMP-2)xa0Ref   Thies RS. Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells. Endocrinology 130: 1318-1324, 1992. PubMed: 1311236 |
| Cellular Products： | alkaline phosphatase induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) Ref Thies RS. Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells. Endocrinology 130: 1318-1324, 1992. PubMed: 1311236 |
| Comments： | Cells used in the W-20-17 assay should not be carried in continuous culture. A working cell bank should be prepared with approximately 5xa0x 105 cell/mL. Cells should be thawed before each assay. Recombinant human bone morphogenetic protein-2 (rhBMP-2) increased alkaline phosphatase activity in W-20-17 cells in a dose-responsive manner without an effect on proliferation.xa0Ref |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Subculture every 3 to 4 days.
Subcultivation Ratio: 1:4 to 1:6 Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Culture medium 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | Genetics Institute, Inc. |
| Deposited As： | mouse |
| References： | Wang W, et al. Ski represses bone morphogenic protein signaling in Xenopus and mammalian cells. Proc. Natl. Acad. Sci. USA 97: 14394-14399, 2000. PubMed: 11121043 Thies RS. Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells. Endocrinology 130: 1318-1324, 1992. PubMed: 1311236 Alden TD, et al. In vivo endochondral bone formation using a bone morphogenetic protein 2 adenoviral vector. Hum. Gene Ther. 10: 2245-2253, 1999. PubMed: 10498255 Bahamonde ME, Lyons KM. BMP3: to be or not to be a BMP.. J. Bone Joint Surg. Am. 83-A: S56-S62, 2001. PubMed: 11263666 Standard Test Method for In Vitro Biological Activity of Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) Using the W-20 Mouse Stromal Cell Line. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 2131-02 (Reapproved 2007)e1. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |