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微信小章| 产品名称： | WEHI 7.1 |
|---|---|
| 商品货号： | TS210685 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Cell Type： | T lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | BALB/c |
| Applications： | Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse. Sensitive to both cortisol and dexamethasone. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse. |
| Antigen Expression： | H-2d; Thy-1.2 |
| Genes Expressed： | H-2d; Thy-1.2 |
| Comments： | Derived from the WEHI 7 thymoma induced by X-irradiation of a BALB/c mouse. Sensitive to both cortisol and dexamethasone. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition of fresh medium or
replacement of medium. Alternatively, cultures can be
established by centrifugation with subsequent resuspension in fresh medium at 2- 3 x 10 exp5 viable cells/ml. A saturation density of 1-2 X 10 exp6 is obtainable. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | AW Harris |
| Deposited As： | Mus musculus |
| References： | Pfahl M, et al. General features of steroid resistance in lymphoid cell lines. Mol. Cell. Endocrinol. 10: 193-207, 1978. PubMed: 566227 Harris AW, et al. Differentiated functions expressed by cultured mouse lymphoma cells. II. theta Antigen, surface immunoglobulin and a receptor for antibody in cells of a thymoma cell line. J. Immunol. 110: 431-438, 1973. PubMed: 4630643 Bourgeois S, Newby RF. Diploid and haploid states of the glucocorticoid receptor gene of mouse lymphoid cell lines. Cell 11: 423-460, 1977. PubMed: 560913 Pfahl M, et al. Interaction of glucocorticoid receptors from lymphoid cell lines with their nuclear acceptor sites. Mol. Cell. Endocrinol. 10: 175-191, 1978. PubMed: 26620 |