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微信小章| 产品名称： | WEHI 164 |
|---|---|
| 商品货号： | TS210686 |
| Organism： | Mus musculus, mouse |
| Cell Type： | methylcholanthrene induced |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrosarcoma |
| Strain： | BALB/c |
| Applications： | These cellsxa0can be used as target cells for short term chromium release cytotoxicity assays. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line was originally established by M. Rollinghoff and N.L. Warner from a fibrosarcoma induced by subcutaneous injections of 3-methylcholanthrene. |
| Comments： | After pretreatment with actinomycin D, the cells are highly sensitive to human cytotoxic monocytes, to human tumor necrosis factor and to lymphotoxin.
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Remove medium, add fresh Trypsin-EDTA Solution, 1X (ATCC® 30-2101™) for 1 to 2 minutes. Remove trypsin and place the flask at 37°C until the cells detach (about 3 to 5 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:20 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | TW LeBien |
| Deposited As： | Mus musculus |
| References： | Ziegler-Heitbrock HW, Riethmuller G. A rapid assay for cytotoxicity of unstimulated human monocytes. J. Natl. Cancer Inst. 72: 23-29, 1984. PubMed: 6582301 Ziegler-Heitbrock HW, et al. Killer cell activity of human monoblastic leukemia cells as detected with a monocyte-specific target cell. Blood 65: 8-14, 1985. PubMed: 3155490 Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111 Rollinghoff M, Warner NL. Specificity of in vivo tumor rejection assessed by mixing immune spleen cells with target and unrelated tumor cells. Proc. Soc. Exp. Biol. Med. 144: 813-818, 1973. PubMed: 4543620 Knowlton KU, et al. A mutation in the puff region of VP2 attenuates the myocarditic phenotype of an infectious cDNA of the woodruff variant of coxsackievirus B3. J. Virol. 70: 7811-7818, 1996. PubMed: 8892902 |