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| 产品名称： | WEHI 22.1 |
|---|---|
| 商品货号： | TS210687 |
| Organism： | Mus musculus, mouse |
| Tissue： | thymus |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | lymphoma |
| Strain： | BALB/c |
| Applications： | The line was derived from a thymoma induced by X-irradiation. They are sensitive to 100 nM cortisol. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The line was derived from a thymoma induced by X-irradiation. |
| Antigen Expression： | Thy-1.2 |
| Genes Expressed： | immunoglobulin; monoclonal antibody,Thy-1.2 |
| Cellular Products： | immunoglobulin; monoclonal antibody |
| Comments： | The line was derived from a thymoma induced by X-irradiation. The cells have characteristics of both T and B cells and form rosettes with sheep erythrocytes. They are sensitive to 100 nM cortisol. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM (surface) |
| Name of Depositor： | AW Harris |
| Deposited As： | Mus musculus |
| References： | Harris AW, et al. Differentiated functions expressed by cultured mouse lymphoma cells. II. theta Antigen, surface immunoglobulin and a receptor for antibody in cells of a thymoma cell line. J. Immunol. 110: 431-438, 1973. PubMed: 4630643 |