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| 产品名称： | VERO C1008 Vero 76, clone E6, Vero E6 |
|---|---|
| 商品货号： | TS210688 |
| Organism： | Cercopithecus aethiops |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Applications： | VERO C1008 exhibits some degree of contact inhibition after forming a monolayer and is therefore useful in growing slow replicating viruses. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line is a clone of VERO 76 (ATCC CRL-1587). It was cloned by the dilution method into microtiter plates in 1979 by P.J. Price.xad Plaques are also produced. |
| Virus Susceptibility： | Junin virus
Machupo virus Lassa virus Marburg virus Zaire Ebola virus |
| Comments： | When infected with the hemorrhagic fever viruses Machupo (Bolivian), Junin (Argentinian), Lassa (African), Marburg or Ebola viruses, these cells exhibit cytopathic effects. Plaques are also produced.xa0 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Time： | 22 hours |
| Name of Depositor： | EM Earley |
| Deposited As： | Cercopithecus aethiops |
| Year of Origin： | 1979 |
| References： | Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988 Schuster FL, Visvesvara GS. Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals. J. Clin. Microbiol. 34: 385-388, 1996. PubMed: 8789020 |