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微信小章| 产品名称： | VA-ES-BJ |
|---|---|
| 商品货号： | TS210690 |
| Organism： | Homo sapiens, human |
| Tissue： | derived from metastatic site: bone marrow |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | epithelioid sarcoma |
| Age： | 41 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | near triploid karyotype with structural as well as numerical abnormalities; modal number = 65. Rearrangements of both chromosomes 1 and 9 with unknown materials were present., Five copies of chromosome 7 were seen in almost every cell, and there was no intact Y chromosome present. Cell to cell variation was due to numerical differences only, structural abnormalities were very consistent. |
| Derivation： | VA-ES-BJ is an epithelioid sarcoma cell line derived in 1991 from a bone marrow metastasis removed from an adult male with primary vertebral epithelioid carcinoma. |
| Clinical Data： | 41 years
male
Caucasian |
| Oncogene： | p53 + |
| Genes Expressed： | Granulocyte macrophage colony stimulating factor (GM-CSF) |
| Cellular Products： | Granulocyte macrophage colony stimulating factor (GM-CSF) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | The cells are sensitive to hemin. The cell are positive for immunohistochemical staining for the following: AE-1 and AE-3 cytokeratins, fas, epithelial membrane antigen (EMA), vimentin and p53. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4.5 g/L glucose and 0.1 mM non-essential amino acids; fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Name of Depositor： | C Helson |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1991 |
| References： | Helson C, et al., Va-es-bj - an epithelioid sarcoma cell-line. Int. J. Oncol. 7: 51-56, 1995. PubMed: 21552805 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |