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| 产品名称： | VD-10 |
|---|---|
| 商品货号： | TS210692 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with a 250000 dalton polypeptide that is part of the saxitoxin binding sodium channel protein in native electroplex membrane. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Animals were immunized with purified saxitoxin binding component of electric eel (E. electricus) electroplex membrane. Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against saxitoxin binding component of electroplex membrane |
| Cellular Products： | immunoglobulin; monoclonal antibody; against saxitoxin binding component of electroplex membrane |
| Comments： | Animals were immunized with purified saxitoxin binding component of electric eel (E. electricus) electroplex membrane. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a 250000 dalton polypeptide that is part of the saxitoxin binding sodium channel protein in native electroplex membrane. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | RPMI 1640 medium, 80%; fetal bovine serum, 20%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | LC Fritz, JP Brockes |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Moore HP, et al. Isolation and characterization of a monoclonal antibody against the saxitoxin-binding component from the electric organ of the eel Electrophorus electricus. Proc. Natl. Acad. Sci. USA 79: 1673-1677, 1982. PubMed: 6280195 |