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微信小章| 产品名称： | V79-4 |
|---|---|
| 商品货号： | TS210693 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | lung |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | male |
| Applications： | Used in studies of X-ray induced damage and repair processes. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 22; range = 20 to 23, Pseudodiploid. The rate of higher ploidies was 4%. Twelve to fourteen marker chromosomes were common to most cells. These include 1p-, 4q+, 4p+, t(6,?), 7q+ and seven to eight other small markers., Normal N2 and N3 were paired; N1, N5, N6 and N10 were single. Normal X and Y were absent, but a single Xq- was present in every cell. |
| Derivation： | In 1966, E.H.Y. Chu obtained the line from W. Sinclair, and isolated the V79-4 clone. This cell line was developed by Ford and Yerganian in 1958 from lung tissue of a young male Chinese hamster, and was originally designated Strain V. A culture at passage 7 after cloning was submitted to the ATCC in July 1988. |
| Clinical Data： | male
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| Comments： | The cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours.
The line was renamed V79 by Elkind in 1958. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:14 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: , Air: 95% CO2: 5% |
| Name of Depositor： | EH Chu |
| Deposited As： | Cricetulus griseus |
| Passage History： | A culture at passage 7 after cloning was submitted to the ATCC in July 1988. |
| Year of Origin： | 1958 |
| References： | . Molecular cell genetics. New York: Wiley; 1985. Chu EH, et al. Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro. Genetics 62: 359-377, 1969. PubMed: 5392645 Chu EH, Malling HV. Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro. Proc. Natl. Acad. Sci. USA 61: 1306-1312, 1968. PubMed: 5249812 . . Nature 184: 1293-1295, 1959. Ford DK, Yerganian G. Observations on the chromosomes of Chinese hamster cells in tissue culture. J. Natl. Cancer Inst. 21: 393-425, 1958. PubMed: 13576097 . Molecular cell genetics. New York: Wiley; 1985. Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 |