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微信小章| 产品名称： | UMR-108 |
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| 商品货号： | TS210698 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | bone |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | osteosarcoma |
| Strain： | Sprague-Dawley |
| Applications： | The cells are responsive to PTH, prostaglandins and bone resorbing steroids. |
| Derivation： | The UMR-108 cell line is a clonal derivative of a transplantable rat osteosarcoma that had been induced by injection of radiophosphorous (32P). Both the original sarcoma and the cloned line were developed by T.J. Martin at the University of Sheffield. |
| Receptor Expression： | parathyroid hormone (PTH); 1-25(OH)2D3 (bone resorbing steroid hormone) |
| Comments： | The UMR-108 cell line is a clonal derivative of a transplantable rat osteosarcoma that had been induced by injection of radiophosphorous (32P). The cells are responsive to PTH, prostaglandins and bone resorbing steroids. The PTH responsiveness of UMR-106 is less than that of the related cell line UMR-106 (ATCC CRL-1661). Both the original sarcoma and the cloned line were developed by T.J. Martin at the University of Sheffield. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: 2 to 3 times per week Remove medium, add fresh 0.25% trypsin for 2 to 3 minutes, remove trypsin and let the culture stand at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor： | AE Bogden |
| Deposited As： | Rattus sp. |
| References： | . Endocrinology of calcium metabolism. Amsterdam: Elsevier/North Holland; 1977. Atkins D, et al. Rat osteogenic sarcoma cells: modulation of hormone stimulated cyclic AMP production by prostaglandin antagonists and biosynthesis inhibitors. Clin. Exp. Pharmacol. Physiol. 7: 31-44, 1980. PubMed: 6103768 Manolagas SC, Deftos LJ. Cytoreceptor assay for 1,25-dihydroxyvitamin D3: a novel radiometric method based on binding of the hormone to intracellular receptors in vitro. Lancet 2: 401-402, 1980. PubMed: 6105521 Partridge NC, et al. Receptors for 1,25(OH)2-vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells. FEBS Lett. 115: 139-142, 1980. PubMed: 6248375 Ingleton PM, et al. Alkaline phosphatase in serum and tumour of rats bearing a hormone- responsive transplantable osteogenic sarcoma. Eur. J. Cancer 15: 685-691, 1979. PubMed: 292595 Crawford A, et al. Membranes from a transplantable osteogenic sarcoma responsive to parathyroid hormone and prostaglandins: regulation of adenylate cyclase and of hormone metabolism. J. Endocrinol. 77: 213-224, 1978. PubMed: 275436 Atkins D, Martin TJ. Rat osteogenic sarcoma cells:effects of some prostaglandins, their metabolites and analogues on cyclic AMP production. Prostaglandins 13: 861-871, 1977. PubMed: 194286 Martin TJ, et al. Parathyroid hormone-responsive adenylate cyclase in induced transplantable osteogenic rat sarcoma. Nature 260: 436-438, 1976. PubMed: 1062678 Ingleton PM, et al. Radiation induced osteogenic sarcoma in the rat as a model of hormone-responsive differentiated cancer. Lab. Anim. Sci. 27: 748-756, 1977. PubMed: 338978 Atkins D, et al. Rat osteogenic sarcoma cells: isolation and effects of hormones on the production of cyclic AMP and cyclic GMP. Endocrinology 101: 555-562, 1977. PubMed: 195798 Crawford A, et al. Rat osteogenic sarcoma cells: comparison of the effects of prostaglandins E1, E2, I2 (prostacyclin), 6-keto F1alpha and thromboxane B2 on cyclic AMP production and adenylate cyclase activity. Biochem. Biophys. Res. Commun. 82: 1195-1201, 1978. PubMed: 212039 . . Clin. Orthop. Relat. Res. 140: 247-254, 1979. Manolagas SC, et al. 1,25-Dihydroxyvitamin D3 receptor-like macromolecule in rat osteogenic sarcoma cell lines. J. Biol. Chem. 255: 4414-4417, 1980. PubMed: 6929275 Underwood JC, et al. Structural and functional correlations in parathyroid hormone responsive transplantable osteogenic sarcomas. Eur. J. Cancer 15: 1151-1158, 1979. PubMed: 294355 . . Trans. Biochem. Sic. 6: 239-241, 1978. Partridge NC, et al. Morphological and biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin. Cancer Res. 43: 4308-4315, 1983. PubMed: 6575864 Martin TJ, et al. Hormonal influences on bone cells. Methods Enzymol. 145: 324-336, 1987. PubMed: 3474491 Forrest SM, et al. Characterization of an osteoblast-like clonal cell line which responds to both parathyroid hormone and calcitonin. Calcif. Tissue Int. 37: 51-56, 1985. PubMed: 3922597 |