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微信小章| 产品名称： | UACC-812 |
|---|---|
| 商品货号： | TS210710 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ductal carcinoma |
| Age： | 43 years |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | range = 58 to 64; abnormal banding region in 3p |
| Derivation： | This cell line was established by A. Liebovitz and associates in 1986 from breast tissue removed at mastectomy to remove a ductal carcinoma (stage II, grade IV). |
| Clinical Data： | female Prior to surgery, the patient had received extensive chemotherapy. |
| Receptor Expression： | estrogen receptor (negative), progesterone receptor (negative) |
| Comments： | Prior to surgery, the patient had received extensive chemotherapy. The cells exhibit a 15 fold amplification of the HER-2/neu oncogene sequence. They are negative for estrogen receptors, progesterone receptors and P glycoprotein. |
| Complete Growth Medium： | Leibovitzs L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Note: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/mL) to the medium. |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 8, 11 D5S818: 11, 12 D7S820: 9 THO1: 9 TPOX: 8, 11 vWA: 16 |
| Population Doubling Time： | 100 hrs |
| Name of Depositor： | A Liebovitz, J Trent |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1986 |
| References： | Proc. Am. Assoc. Cancer Res. 29: 24, 1988. Meltzer P, et al. Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. Br. J. Cancer 63: 727-735, 1991. PubMed: 1674877 |