微信小陈
微信小章
产品简介
购买须知
| 产品名称： | UC3-10A6 |
|---|---|
| 商品货号： | TS210713 |
| Organism： | Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the murine T cell receptor gamma/delta TCR. The antibody is specific for receptors containing the V gamma 2 segment. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR) |
| Comments： | Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the murine T cell receptor gamma/delta TCR. The antibody is specific for receptors containing the V gamma 2 segment. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG |
| Name of Depositor： | JA Bluestone |
| Deposited As： | hamster, Armenian (B cell); mouse (myeloma) |
| References： | Dent AL, et al. Self-reactive gamma delta T cells are eliminated in the thymus. Nature 343: 714-719, 1990. PubMed: 1968227 |