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微信小章| 产品名称： | U-87 MG |
|---|---|
| 商品货号： | TS210714 |
| Organism： | Homo sapiens, human |
| Tissue： | brain |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Likely glioblastoma |
| Age： | unknown |
| Gender： | male |
| Ethnicity： | unknown |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypodiploid human cell line with the modal chromosome number of 44 occurring in 48% of cells. The rate of higher ploidy was 5.9%., Twelve markers were common to all cells, including der(1)t(1;3) (p22;q21), der(16)t(1;16) (p22;p12), del(9) (p13) and nine others. The marker der(1) had two copies in most cells. There was only one copy of normal X. N1, N6 and N9 were not found. |
| Images： |  |
| Derivation： | This is one of a number of cell lines derived from malignant gliomas (see alsoxa0ATCC®xa0HTB-15™xa0andxa0HTB-16™)xa0by J. Ponten and associates from 1966 to 1969 (see also Allen, 2016). |
| Clinical Data： | male |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice inoculated subcutaneously with 10(7) cells |
| Comments： | Mycoplasma contamination was eliminated in September 1975.
The ATCC®xa0HTB-14™xa0cell line was deposited at ATCC in 1982.
STR profiling, Y-chromosome paint, and Q-band assay confirmed that thexa0cell line is male in origin. Based on current literature, the cell line is likely a glioblastoma of CNS origin (Allen, 2016). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75xa0cm2xa0flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: 5% CO2 in air recommended
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,11
D7S820: 8,9
D5S818: 11,12
D16S539: 12
vWA: 15,17
THO1: 9.3
TPOX: 8 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 2 PGM3, 1 |
| Name of Depositor： | J Ponten |
| Deposited As： | Homo sapiens |
| Year of Deposit： | 1966 |
| References： | Allen M, et al. Origin of the U87MG glioma cell line: Good news and bad news. Sci. Trans. Med. 8(354): 1-4, 2016. Clark MJ, et al. U87MG decoded: The genomic sequence of a cytogenetically aberrant human cancer cell line. PLoS Genetics 6 (1) : e1000832, 2010. Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306 Olopade OI, et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744 Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504 Tumors developed within 21 days at 100% frequency (5/5). |
| Cross References： | Nucleotide (GenBank) : E02404 DNA encoding exon K of NAP. Nucleotide (GenBank) : X06981 Human mRNA fragment for amyloid beta-protein (AP) insertion. Nucleotide (GenBank) : E02405 DNA encoding PI(the active region that has protease inhibitor activity of NAP). Nucleotide (GenBank) : E02400 DNA encoding NAP(new senile plaque amyloid precursor protein having protease inhibitor). Nucleotide (GenBank) : E02402 DNA encoding exon I of NAp(new senile plaque amyloid precursor having protease inhibitor). Nucleotide (GenBank) : E02401 DNA encoding exon H of NAp(new senile plaque amyloid precursor protein having protease inhibitor). Nucleotide (GenBank) : E02403 DNA encoding exon J of NAP(new senile plaque amyloid precursor protein having protease inhibitor). |