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微信小章| 产品名称： | UC7-13D5 |
|---|---|
| 商品货号： | TS210715 |
| Organism： | Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the murine T cell receptor gamma/delta TCR. The antibody is specific for all murine TCR gamma/delta heterodimers. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR) |
| Comments： | Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the murine T cell receptor gamma/delta TCR. The antibody is specific for all murine TCR gamma/delta heterodimers. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Medium Renewal: Two to three times weekly. |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG |
| Name of Depositor： | JA Bluestone |
| Deposited As： | hamster, Armenian (B cell); mouse (myeloma) |
| References： | Bluestone JA, et al. Repertoire development and ligand specificity of murine TCR gamma delta cells. Immunol. Rev. 120: 5-33, 1991. PubMed: 1650760 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |