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| 产品名称： | TSHR-T3-365 |
|---|---|
| 商品货号： | TS210718 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor. |
| Derivation： | Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, intracellular domain |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor beta subunit, intracellular domain |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in BALB/c mice |
| Comments： | Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the C-terminal intracellular domain (amino acids domain 604-764), beta subunit of the human thyroid stimulating hormone (TSH) receptor. It recognizes an intracellular epitope of the beta subunit anchored into the cellular membrane. The TSHR-R5T-34 (ATCC CRL-2683) antibody recognizes a N-terminal extracellular epitope of the beta subunit while the TSHR-T3-365 (TS210718) antibody recognizes a C-terminal intracellular subunit of the beta subunit. The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
|
| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | H Loosfelt, E Milgrom |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Loosfelt H, et al. Two-subunit structure of the human thyrotropin receptor. Proc. Natl. Acad. Sci. USA 89: 3765-3769, 1992. PubMed: 1570295 |