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微信小章| 产品名称： | TUR |
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| 商品货号： | TS210724 |
| Organism： | Homo sapiens, human |
| Tissue： | histiocytic lymphoma |
| Cell Type： | histocyte |
| Product Format： | frozen |
| Morphology： | monocyte |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | histiocytic lymphoma |
| Age： | 37 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Derivation： | TUR (TPA-U937-Resistant) is a stably transfected cell line generated by Ralf Hass and Masanori Hirano in 1992 from U-937 (ATCC CRL-1593.2), a monoblastoid cell line. U-937 cells were transfected by electroporation with the pMG2neoPA plasmid containing the neomycin resistant gene and then selected in medium containing G418 and 12-O-tetradecanoylphorbol-13-acetate (TPA). |
| Clinical Data： | 37 years Caucasian, White male |
| Antigen Expression： | HLA DR |
| Comments： | TUR cells have remained resistant to both G418 and TPA after culture in the absence of these agents for over 100 passages.
The cell line retains monocytic properties but does not differentiate along the macrophage pathway by phorbal ester treatment. While treatment of human U-937 myeloid leukemia cells with TPA is associated with growth arrest and induction of monocytic differentiation, the TUR cell line is unresponsive to the growth-inhibitory effects of this agent. The TUR variant is defective in TPA-induced signaling events upstream to activation of Raf-1 kinase. Expression of major histocompatibility complex (MHC) Class II antigens on U937 and TPA-treated U937 cells is barely detectable.
However, there was a significantly constitutive expression of MHC class II, particularly human lymphocyte antigen (HLA-DR) on the surface of TUR and TPA-treated TUR cells. TUR cells continue to proliferate in the presence of TPA although increasing concentrations of TPA continuously reduces the proliferative capacity of the cells.
Unlike U-937 cells, exposure to TPA did not induce detectable levels of internucleosomal DNA fragmentation or the generation of apoptotic bodies.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 2 x 106 cells/mL. Do not allow the cell concentration to exceed 2 X 106 cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 10,12 D16S539: 12 D5S818: 12 D7S820: 9,11 THO1: 6,9.3 TPOX: 8,11 vWA: 14,16 |
| Name of Depositor： | R Hass, I Prudovsky |
| Passage History： | TUR cells have remained resistant to both G418 and TPA after culture in the absence of these agents for over 100 passages. |
| References： | Hass R, et al. Resistance to phorbol ester-induced differentiation of a U-937 myeloid leukemia cell variant with a signaling defect upstream to Raf-1 kinase. Cell Growth Differ. 4: 657-663, 1993. PubMed: 8398907 Hass R, et al. Characterization of human TUR leukemia cells: continued cell cycle progression in the presence of phorbol ester is associated with resistance to apoptosis. Eur. J. Cell Biol. 65: 408-416, 1994. PubMed: 7720732 Hass R, Lopez-Guerrero JA. Aggressive tumor growth of human TUR leukemia cells is associated with high levels of c-myc expression and down-regulation of p20-max. Int. J. Cancer 72: 1113-1116, 1997. PubMed: 9378547 Meinhardt G, et al. Signaling defect in the activation of caspase-3 and PKCdelta in human TUR leukemia cells is associated with resistance to apoptosis. Exp. Cell Res. 247: 534-542, 1999. PubMed: 10066381 |