微信小陈
微信小章| 产品名称： | U-2 OS |
|---|---|
| 商品货号： | TS210726 |
| Organism： | Homo sapiens, human |
| Tissue： | bone |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | osteosarcoma |
| Age： | 15 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This cell line is a suitable transfection host. |
| Karyotype： | Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen. Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::?), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others.
Ref  Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590 |
| Images： |  |
| Derivation： | J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl. |
| Clinical Data： | 15 years Caucasian female J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl. |
| Antigen Expression： | Antigen expression: Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
| Receptor Expression： | Receptor expression: insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II) |
| Genes Expressed： | osteosacoma derived growth factor (ODGF),Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
| Comments： | Viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses. Mycoplasma contamination was detected and eliminated in 1972. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product. Subcultivation Ratio:xa0A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal:xa02 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 13 D13S317: 13 D16S539: 11,12 D5S818: 11 D7S820: 11,12 THO1: 6,9.3 TPOX: 11,12 vWA: 14,18 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 PGM1, 2 PGM3, 1 |
| Name of Depositor： | Hellstrom |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1964 |
| References： | Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080 Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590 Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159 : 531-541 , 1994. PubMed: 8188767 Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029 Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487 Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590 |