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微信小章| 产品名称： | U266B1 U266 |
|---|---|
| 商品货号： | TS210727 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | myeloma; plasmacytoma |
| Age： | 53 years adult |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Images： |  |
| Clinical Data： | male |
| Genes Expressed： | immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6) |
| Cellular Products： | immunoglobulin; monoclonal antibody; interleukin 6 (interleukin-6, IL-6) |
| Comments： | U266 cells have been reported to produce human IL-6. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.xa0Corning® T-75 flasks (catalog #431464) are recommended for subculturing this product. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 12,13 D13S317: 12 D16S539: 10 D5S818: 11,12 D7S820: 11,12 THO1: 5,7 TPOX: 8 vWA: 17 |
| Isotype： | IgE; lambda light chain |
| Name of Depositor： | NK Nilsson |
| Deposited As： | Homo sapiens |
| References： | Nilsson K, et al. Established immunoglobulin producing myeloma (IgE) and lymphoblastoid (IgG) cell lines from an IgE myeloma patient. Clin. Exp. Immunol. 7: 477-489, 1970. PubMed: 4097745 Kawano M, et al. Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature 332: 83-85, 1988. PubMed: 3258060 Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 |