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| 产品名称： | tsc2 ang1 |
|---|---|
| 商品货号： | TS210737 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | cutaneous sarcoma |
| Applications： | The tsc2 ang1 cell line was derived in 1998 from a cutaneous sarcoma taken from a Tsc2+/- mouse heterozygous for tuberin. It can be used for drug testing and for angiogenesis studies. The cell line is a useful model of TSC. It is the only genetically defined tuberous sclerosis cell line available that forms tumors. |
| Derivation： | Tuberous sclerosis (TSC) is an autosomal dominant genetic disorder caused by mutations in either TSC1 or TSC2. Mice heterozygous for Tsc2 were developed as a murine model of Tsc2 disease using a gene targeting approach. The tsc2 ang1 cell line was derived in 1998 from a cutaneous sarcoma taken from a Tsc2+/- mouse heterozygous for tuberin. |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | Tuberous sclerosis (TSC) is an autosomal dominant genetic disorder caused by mutations in either TSC1 or TSC2. Mice heterozygous for Tsc2 were developed as a murine model of Tsc2 disease using a gene targeting approach. The tsc2 ang1 cell line was derived in 1998 from a cutaneous sarcoma taken from a Tsc2+/- mouse heterozygous for tuberin. The cell line is a useful model of TSC. It is the only genetically defined tuberous sclerosis cell line available that forms tumors. It can be used for drug testing and for angiogenesis studies. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:5 is recommended Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Name of Depositor： | JL Arbiser |
| Deposited As： | mouse |
| Year of Origin： | 1998 |
| References： | Onda H, et al. Tsc2(+/-) mice develop tumors in multiple sites that express gelsolin and are influenced by genetic background. J. Clin. Invest. 104: 687-695, 1999. PubMed: 10491404 |