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微信小章| 产品名称： | TM4 |
|---|---|
| 商品货号： | TS210739 |
| Organism： | Mus musculus, mouse |
| Tissue： | testis |
| Cell Type： | Sertoli cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 11 to 13 days |
| Gender： | male |
| Applications： | transfection host |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Antigen Expression： | H-Y antigen; Mus musculus, expressed |
| Receptor Expression： | follicle stimulating hormone (FSH), expressed androgen receptor, expressed progesterone receptor, expressed |
| Genes Expressed： | retinol binding protein, tissue plasminogen activator, transferrin |
| Tumorigenic： | No |
| Effects： | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone (LH). The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | A 1:1 mixture of HamS F12 medium and Dulbeccos modified Eagles medium with 1.2 g/L sodium bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:50 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | JP Mather |
| Deposited As： | Mus musculus |
| References： | Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781 Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561 Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells. J. Biol. Chem. 259: 3117-3123, 1984. PubMed: 6538197 Mather JP, Phillips DM. Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture. J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874 |