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| 产品名称： | TM1 |
|---|---|
| 商品货号： | TS210740 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 BALB/c (B cell); BALB/c (myeloma) |
| Applications： | Animals were immunized with human leukocytes (HLA phenotype A2,A28,Bw4,B27,B44,Cw2). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Leu-5; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Leu-5; cytotoxic |
| Comments： | Animals were immunized with human leukocytes (HLA phenotype A2,A28,Bw4,B27,B44,Cw2). Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for Leu-5 positive (Leu-5+) cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Modified RPMI 1640 medium (see fact sheet), 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM |
| Name of Depositor： | F Grumet |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Grumet FC, et al. A simplified new method for HLA-DR typing using the TM1 monoclonal antibody. Hum. Immunol. 6: 63-73, 1983. PubMed: 6601099 |