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微信小章| 产品名称： | TOV-112D |
|---|---|
| 商品货号： | TS210741 |
| Organism： | Homo sapiens, human |
| Tissue： | Ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | grade 3, stage IIIC, primary malignant adenocarcinoma; endometrioid carcinoma |
| Age： | 42 years adult |
| Gender： | female |
| Storage Conditions： | liquid nitrogen vapor temperature |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype： | 52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17
Ref  Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 |
| Images： |  |
| Derivation： | This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer. |
| Clinical Data： | female This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. |
| Oncogene： | her2/neu +, p53 (mutated, Arg --> His mutation at exon 6, codon 175)
RefProvencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 |
| Genes Expressed： | keratin |
| Cellular Products： | keratin |
| Tumorigenic： | Yes |
| Effects： | The cells are tumorigenic in nude mice and form colonies in soft agar |
| Comments： | Unlike TOV-21G (ATCC CRL-11730) and OV-90 (ATCC CRL-11732), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24. |
| Complete Growth Medium： | The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate.
To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 8 D16S539: 9,12 D5S818: 10 D7S820: 9,10 THO1: 6 TPOX: 8,11 vWA: 18 |
| Name of Depositor： | University of Montreal |
| Deposited As： | human |
| U.S. Patent Number： | |
| Year of Origin： | 1992 |
| References： | Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998 Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993 |