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| 产品名称： | Toledo |
|---|---|
| 商品货号： | TS210742 |
| Organism： | Homo sapiens, human |
| Tissue： | peripheral blood |
| Cell Type： | B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | diffuse large cell lymphoma; non-Hodgkins B cell lymphoma |
| Age： | adult |
| Gender： | female |
| Ethnicity： | White |
| Applications： | This cell line is a model system for studying non-Hodgkin lymphomas. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Toledo was established in 1990 from peripheral blood leukocytes (PBL) of a patient that originally had a diffuse large cell lymphoma (DLCL). |
| Clinical Data： | adult
female
White |
| Antigen Expression： | CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - Ref   Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |
| Comments： | Following high-dose chemotherapy and bone marrow transplantation, the patient subsequently developed a lymphoma in the brain. Though the morphology of the cells resembles Burkitts lymphoma, the cells lack the typical chromosomal translocations of Burkitts lymphoma. The karyotype does exhibit multiple chromosomal aberrations. The cells do not express surface or cytoplasmic immunoglobulin. The cells are negative for Epstein-Barr virus. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 105 viable cells/mL. Maintain cultures at cell concentrations between 3 x 105 and 3 x 106 viable cells/mL. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | D5S818:10,12 D7S820: 9,10 THO1: 8,9.3 TPOX: 8,11 vWA: 16,17 Amelogenin: X CSF1PO: 12 D13S317:11 D16S539:9 |
| Population Doubling Time： | 24 to 30 hrs |
| Name of Depositor： | H Ben-Bassat |
| Deposited As： | human |
| Year of Origin： | 1990 |
| References： | Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkins lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273 |