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| 产品名称： | TM3 |
|---|---|
| 商品货号： | TS210744 |
| Organism： | Mus musculus, mouse |
| Tissue： | testis |
| Cell Type： | Leydig cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 11 to 13 days |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Clinical Data： | male |
| Receptor Expression： | luteinizing hormone (LH); epidermal growth factor (EGF); androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive |
| Genes Expressed： | prostaglandin F2a |
| Tumorigenic： | No |
| Effects： | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | The TM3 cell line responds to LH with an increase in cAMP production, but does not respond to follicle stimulating hormone (FSH). The maintenance of responsiveness to LH is dependent upon serum lot. In the presence of LH, the cells are capable of metabolizing cholesterol. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | A 1:1 mixture of Hams F12 medium and Dulbeccos modified Eagles medium with 2.5mM L-Glutamine, 0.5mM Sodium Pyruvate, 1.2 g/L Sodium Bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin-0.53mM EDTA solution, rinse, remove trypsin and let the culture sit at room temperature for 2 to 4 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:20 to 1:200 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%, DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Name of Depositor： | JP Mather |
| Deposited As： | Mus musculus |
| References： | Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781 Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561 |